2015 / May / mRNAseq on non-model organisms¶
This workshop was given on May 4th and 5th, 2015, by C. Titus Brown, Camile Scott, and Tamer Mansour. See the workshop organization page for more information, or contact Titus directly.
Tutorials:
- Welcome!
- Non-model organisms and RNAseq
- Getting started with Amazon EC2
- Short read quality and trimming
- Running digital normalization
- Running the actual assembly
- BLASTing your assembled data
- Annotation of denovo transcriptome
- Quantification and Differential Expression
- Remapping your reads to your assembled transcriptome
- Miscellaneous advice
- More resources
- Miscellaneous questions
- Tips and Tricks for working with Remote Computers
Technical information¶
The github repository for this workshop is publicly available at https://github.com/ngs-docs/2015-may-nonmodel.
LICENSE: This documentation and all textual/graphic site content is licensed under the Creative Commons - 0 License (CC0) -- fork @ github. Presentations (PPT/PDF) and PDFs are the property of their respective owners and are under the terms indicated within the presentation.